The Normal Distribution

Why is the histogram so important?

Due to the fact that random measurement errors always occur, one can observe replicate measurements to differ slightly. This deviation from a central value (usually taken to be the 'true' value) can be summarized by a histogram. A histogram shows the (relative) frequency of each measurement.

Practical results

In the figure below you see the practical results of two chemistry students pipetting a volume of 50 x 200 µL with a P1000 pipet (a pipet with a volume of 1000 µL). As you can see, there is considerable spread in the results. However, values close to the true value of 200 µL are more often found than values far away for student 1. On the other hand, student 2 finds consistently lower values than the other student.

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Question: which results are more precise and which are more accurate?

Why is the normal distribution so important?

Histograms can be very useful to summarize the spread in the data. In many cases, the spread in the data can also be described by a normal distribution. Put differently: random experimental errors show a normal distribution. If the number of experiments is large enough, the histogram approximates the (smoother) shape of a normal distribution.

Location and width of the normal distribution

The normal distribution is characterised by two parameters: the mean and the standard deviation. With these, we can calculate the theoretical normal distribution (or Gauss-curve). When you fill in values for mean and standard deviation, two new plots will appear. In the right plot, the scale of the figure will remain as it is, showing the change of the curve relative to the original situation. The left plot contains the same data but will show you the 'optimally scaled' plot.

  1. What is the effect on the Gauss-curve of changing the mean? Try a few values.
  2. What is the effect of changing the standard deviation? Try a few values.


Theory and Experiment

If we calculate values for the mean and the standard deviation of another data set, containing titration data, we obtain the corresponding Gauss curve shown below:
gausshist
The agreement is not very good, because the number of titrations is quite small. When we perform more experiments, the results will be more like the theoretical distribution. You can see that by simulating a number of experiments and comparing theory and experiment:



  1. Try the same number of experiments several times, and observe the diversity in histograms. All of these data sets are randomly drawn from the same population!
  2. Try different numbers of experiments (minimum 3, maximum 500) and different values for mean and standard deviation.
  3. Can you draw conclusions on the quality of the approximation for small and large sample sizes?

Now, continue with the questions on this subject.

15 thoughts on “The Normal Distribution

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