For a calibration experiment you have prepared a number of standard solutions with increasing concentrations. The correct measurement order is to go from low concentrations to high concentrations, to avoid contamination of the cuvette. If you would go from high to low concentrations, what effect might this have on the calibration line?

The line will be less steep
The line will be shifted to the right with the same slope
The line will be steeper
The line will be shifted to the left with the same slope